Sodium salicylate prevents inflammation-associated decreases in phosphorylated-Enos SER1177 in human aortic endothelial cells through an AMPK-dependent mechanism
نویسندگان
چکیده
ii To my sister, Andrea, who gave me advice and support; and to Dr. Robert Oppliger, who coached and assisted my entry into graduate school.
منابع مشابه
When metabolism rules perfusion: AMPK-mediated endothelial nitric oxide synthase activation.
Since the first description of the AMP-activated protein kinase (AMPK) more than 20 years ago,1 numerous studies have established a pivotal role of this enzyme in the regulation of cellular energy balance. Decreased cellular energy supply (indicated by an increased ATP breakdown and accumulation of AMP) leads to an AMPK-mediated adaptation to allow cellular survival. In doing so, AMPK acts as a...
متن کاملAMP-activated protein kinase functionally phosphorylates endothelial nitric oxide synthase Ser633.
Endothelial nitric oxide synthase (eNOS) plays a central role in maintaining cardiovascular homeostasis by controlling NO bioavailability. The activity of eNOS in vascular endothelial cells (ECs) largely depends on posttranslational modifications, including phosphorylation. Because the activity of AMP-activated protein kinase (AMPK) in ECs can be increased by multiple cardiovascular events, we ...
متن کاملThe role of AMP-activated protein kinase in the functional effects of vascular endothelial growth factor-A and -B in human aortic endothelial cells
BACKGROUND Vascular endothelial growth factors (VEGFs) are key regulators of endothelial cell function and angiogenesis. We and others have previously demonstrated that VEGF-A stimulates AMP-activated protein kinase (AMPK) in cultured endothelial cells. Furthermore, AMPK has been reported to regulate VEGF-mediated angiogenesis. The role of AMPK in the function of VEGF-B remains undetermined, as...
متن کاملAzilsartan, an angiotensin II type 1 receptor blocker, restores endothelial function by reducing vascular inflammation and by increasing the phosphorylation ratio Ser1177/Thr497 of endothelial nitric oxide synthase in diabetic mice
BACKGROUND Azilsartan, an angiotensin II type 1 (AT1) receptor blocker (ARB), has a higher affinity for and slower dissociation from AT1 receptors and shows stronger inverse agonism compared to other ARBs. Possible benefits of azilsartan in diabetic vascular dysfunction have not been established. METHODS We measured vascular reactivity of aortic rings in male KKAy diabetic mice treated with v...
متن کاملModification of endothelial NO synthase through protein phosphorylation after forebrain cerebral ischemia/reperfusion.
BACKGROUND AND PURPOSE Production of NO by endothelial NO synthase (eNOS) is thought to play a neuroprotective role after cerebral ischemia. The vascular endothelial growth factor (VEGF) contributes to activation of eNOS by Ca2+/calmodulin and also stimulates the protein kinase Akt, which directly phosphorylates eNOS on Ser1177 and increases enzyme activity. Although the expression of VEGF has ...
متن کامل